Roche LightCycler 2.0 6-Channel Real Time PCR Complete System

Description:

The LightCycler® 1.3 Instrument allows you to monitor amplification of 32 PCR products simultaneously, in real-time and online, with six different detection channels in multiplex experiments. The LightCycler® Software 4.1 collects fluorescence data from all six channels at each cycle and displays it immediately, enabling you to terminate PCR runs early if signal levels are adequate, or prolong them when signals are too low. Amplification occurs in specially designed reaction capillaries, whose superb surface-to-volume ratio provides extremely rapid thermal transfer, facilitating high-speed thermal cycling. The combination of LightCycler® Capillaries and air-driven temperature control allows an entire 35-cycle run to be performed in as little as 30 minutes (with 20 µl capillaries)
to 60 minutes (with 100 µl capillaries).

Features:

- Obtain fast, highly reproducible results using real-time, online PCR. Assay and analysis are complete in less than 45 minutes.
- Expect a greater signal-to-noise ratio and fewer unwanted PCR products, due to the rapid cycling of the LightCycler® Instruments.
- Choose from a wide variety of fluorescence formats for flexible experimental design and analysis.
- Efficiently manage and protect your data with LightCycler® Software 4.05/4.1 analysis modules.
- Employ sensitive and specific real-time PCR quantification in countless research applications.

Use the LightCycler® 1.3 Instrument to perform:

- Fast and sensitive real-time PCR and RT-PCR
- Reproducible and reliable kinetic quantification of DNA, cDNA, or RNA
- Easy PCR product identification and characterization by melting point analysis
- Highly specific mutation analysis and SNP (single nucleotide polymorphism) genotyping
- Qualitative PCR product detection
- Multiplex assays using the HybProbe detection format
- Convenient and exact determination of nucleic acid concentrations before setting the PCR reaction
- Gene expression analysis
- Gene knockdown result validation
- Food pathogens and spoilage testing
- Screening for genetically modified organisms (GMO)
- Specific analysis of oncological or virological research parameters

Specifications:

• Dimensions (W x D x H): 28.0 cm x 38.5 cm x 50.5 cm; (11 in x 11 in x 20 in)
• Weight: Approximately 22 kg (48.5 lbs)
• Computer: Notebook with Windows XP and pre-installed LightCycler® Software 4.1
• Sample Number: 32
• Reaction Vessel: LightCycler® Capillary
• Reaction Volume: 20 µl or 100 µl
• Fluorescence Excitation: Blue LED with peak emission of 470 nm
• Detection Channels: 6 (530, 560, 610, 640, 670, 710 nm)
• Measuring Mode: Real-time, online (continuous display of readings)
• Typical Run Time: <30 minutes for 35 cycles
• Temperature Ramp Rate (settings): 0.1-20°C/s
• Temperature Accuracy: ± 0.4°C

A Windows Notebook with pre-installed LightCycler® Software 4.1 is supplied with the LightCycler® 1.3 Instrument.

LightCycler® Software 4.1

- Automatically analyzes and interprets real-time data during either amplification or melting curve analysis.
- Analysis modules automatically perform qualitative detection, absolute or relative quantification, mutation detection, and genotyping.
- Automated melting curve analysis facilitates identification of unique melting species and grouping of genotypes.
- Nucleic acid quantification can be done before PCR.
- Macros allow control of several workflow steps with just one keystroke.

Items delivered:

- LightCycler® 1.3 Instrument
- Windows Notebook with pre-installed LightCycler® Software 4.1
- LightCycler® Sample Carousel (100 µl)
- Power Cord
- Connection Cable (RS232)
- Instruction Manual